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2014•Anhui nongye kexueRequires access

Study on the Plant Regeneration Induced from Calli and Regeneration System from Rootstock in vitro

Yanan Zhao

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Abstract

[Objective] The aim was to study the plant regeneration induced from calli and regeneration system from rootstock in vitro.[Method]Vitis riparia and Vitis amurensis × Vitis riparia were used as experiment materials. Using the methods of comparation and positive cross experiment the best explant and the most suitable medium were studied. [Result]The best explant of leaf,petiole stock,stem,root was petiole stock. The most suitable medium for regeneration was MS + 6-BA 3. 0 mg / L + NAA 0. 05 mg / L,and the rate of regeneration was 6. 25%. MS +TDZ 3. 0 mg / L + IAA 0. 05 mg / L also could induce the bud germination,and the rate of regeneration was 2. 5%. [Conclusion] Regeneration system of rootstock was established.

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What this paper is about

[Objective] The aim was to study the plant regeneration induced from calli and regeneration system from rootstock in vitro.[Method]Vitis riparia and Vitis amurensis × Vitis riparia were used as experiment materials. Using the methods of comparation and positive cross experiment the best explant and the most suitable medium were studied. [Result]The best explant of leaf,petiole stock,stem,root was petiole stock. The most suitable medium for regeneration was MS + 6-BA 3. 0 mg / L + NAA 0. 05 mg / L,and the rate of regeneration was 6. 25%. MS +TDZ 3. 0 mg / L + IAA 0. 05 mg / L also could induce the bud germination,and the rate of regeneration was 2. 5%. [Conclusion] Regeneration system of rootstock was established.

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Available abstract

[Objective] The aim was to study the plant regeneration induced from calli and regeneration system from rootstock in vitro.[Method]Vitis riparia and Vitis amurensis × Vitis riparia were used as experiment materials. Using the methods of comparation and positive cross experiment the best explant and the most suitable medium were studied. [Result]The best explant of leaf,petiole stock,stem,root was petiole stock. The most suitable medium for regeneration was MS + 6-BA 3. 0 mg / L + NAA 0. 05 mg / L,and the rate of regeneration was 6. 25%. MS +TDZ 3. 0 mg / L + IAA 0. 05 mg / L also could induce the bud germination,and the rate of regeneration was 2. 5%. [Conclusion] Regeneration system of rootstock was established.

Key concepts: Rootstock, Explant culture, Petiole (insect anatomy), Regeneration (biology), Biology, Botany, Germination, Horticulture

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