Establishment of the Regeneration System of Poplar Leaf Explants

[Objective] The scientific reference for the gene transformation of poplar was provided through the research on the regeneration technique of the poplar(Populus×euramericana cv.'Guariento')leaf culturing.[Method] The effect of the disinfection method and plant growth regulator on the bud growth and the regeneration from leaf culture was experimented.[Results] The best disinfection method was the leaf was treated with 75% alcohol solution for 10 seconds,and then,soaked with 0.1% HgCl2 for 10 minutes,with low contamination and browning rate and high survival rate.The best medium for the inducement of bud was MS+6-BA 0.6 mg/L+NAA 0.2 mg/L with the inducement rate:100% and the average rate of bud-growing:26.43 buds.The best rooting medium was 1/2MS+IBA 0.3 mg/L or 1/2MS+IAA 0.3 mg/L,with the root-growing rate:100% and the average number of root:4.83 and 5.20 roots.[Conclusion] The regeneration system of poplar leaf culturing was established.