2012•Biotechnology(Faisalabad)Requires access

Identification and Phylogenetic Analysis on Endophytic Bacteria Isolated from Taxus chinensis var mairei

LU Zhuo-yue

Abstract

Objective:The endophytic bacteria composition of Taxus chinensis var mairei was investigated.Method:Identification to these isolates was carried out with morphology,biochemistry and molecular biology methods,respectively.The phylogenetic analysis was carried out,too.Result:In total 52 of endophytic bacteria were isolated from Taxus chinensis var mairei.Result:Of 16S rRNA gene amplification by PCR method shown that about 1 400 bp length of DNA fragments were amplified.A comparative analysis of the 16S rRNA gene sequence demonstrated that strain TB02 was closely related to Enterobacter sp.S60(99 % similarity),strain TB13 was closely related to Lysinibacillus sp.E4(97 % similarity),and the other nine strains were to closely related Bacillus genus(99% or 100% similarity).The eleven isolates clustered two main branches in evolutionary tree constructed.Conclusion:Bacillus was the dominant genus.The evolutionary distance between Bacillus and Lysinibacillus was closer than that between them and Enterobacter.

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Key concepts: 16S ribosomal RNA, Biology, Phylogenetic tree, Botany, Taxus, Enterobacter, Bacteria, Strain (injury)