Phylogenetic Analysis of Twenty-five Strains of Endophytic Actinomycetes in Plants

[Objective] The research aimed to confirm the genus correlation among 25 strains of endophytic actinomycetes in plants preliminarily.[Method] The genomic DNA was extracted from 25 strains of endophytic actinomycetes isolated from such plants as wheat,onion and rape.PCR amplification and sequencing were made on its 16S rDNA sequences to make the phylogenetic analysis.[Result] The phylogenetic analysis results showed that 25 strains of endophytic actinomycetes in plants could be divided into 5 genus,including 1 strain of Glycomyces,2 strains of Nocardiopsis,7 stains of Nocardia sp.,10 strains of Micromonospora and 5 strains of Streptomyces.[Conclusion] Among 25 tested strains,the proportion of Micromonospora was highest.The evolutionary distance between the tested endophytic actinomycete and the known strain in the corresponding genus was concentrated in the range of 97.39%～99.92%.