Identif ication and Phylogenetic Analysis of Four Endophytic Bacteria Isolated from Alsophila spinulosa

To determine the endophytic bacteria inhabiting Alsophila spinulosa and their phylogenetic relationship,integrative identification and phylogenetic analysis was made on 4 strains of isolates with traditional microbiological methods.Based on morphological and biochemical properties and sequencing and comparison results with BLAT,the isolates ASP17 and ASL21 were identified as species Bacillus thuringiensis,ASL24 was identified as genus Lysinibacillus,while ASL23 possibly belonged to another genus close to Lysinibacillus.Among the 4 endophytic bacteria,ASP17 and ASL21 clustered in one branch,while ASP23 and ASP24 clustered in another branch in cladogram.The results revealed that the genetic relationship between ASP17 and ASL21 is closer than between ASP23 and ASP24.