Isolation and phylogenetic analysis of endophytic bacteria in Davidia involucrate

A total of 56 endophytic bacteria were isolated from the stem,leaves and petioles of Davidia involucrate with tradational cultural methods.Of which,17 endophytic bateria were selected to carry out succedent molecular studies.About 1 500 bp fragments of 16S rDNA were amplified by PCR method using the extracted genomic DNA as the templet.According to sequences analysis and comparison with the data of GenBank by BLAST,eleven endophytic bacreia were classed to Bacillus genera,the identities are from 95% to 98%,two were classed to Lysinibacillus genera,the identities are 97% and 99%,one were classed to Bordetella genus,the identity was 95%,and one was similar to a reported uncultured bacterium,the identity was 96%.Bacillus was the dominant genus among the endophytic bacteria in Davidia involucrate.The phylogenic tree showed that the 15 endophytic bacteria clustered two main branches.