ScholarLens Back to search
2012•China Animal Husbandry & Veterinary MedicineRequires access

Cloning and Sequence Analysis of Fusion Protein Gene of Canine Distemper Virus Isolated from Guizhou

Zhiyong Zeng

Open publisher page 0 citations

Abstract

Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank,and the Fgene was amplified by reverse transcription polymerase chain reaction(RTPCR) from CDV-GZ1strain.The amplified fragment was cloned and analyzed.The results showed that the length of CDVGZ1 Fgene was 1989bp.It contained five potential N-glycosylation locations based on the derived amino acid sequences.The homology of coding amino acid between this strain and American 00-2601and domestic HL strain was 91.6% and 91.1%respectively,it showed that they had common ancestor.

About this research paper

What this paper is about

Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank,and the Fgene was amplified by reverse transcription polymerase chain reaction(RTPCR) from CDV-GZ1strain.The amplified fragment was cloned and analyzed.The results showed that the length of CDVGZ1 Fgene was 1989bp.It contained five potential N-glycosylation locations based on the derived amino acid sequences.The homology of coding amino acid between this strain and American 00-2601and domestic HL strain was 91.6% and 91.1%respectively,it showed that they had common ancestor.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank,and the Fgene was amplified by reverse transcription polymerase chain reaction(RTPCR) from CDV-GZ1strain.The amplified fragment was cloned and analyzed.The results showed that the length of CDVGZ1 Fgene was 1989bp.It contained five potential N-glycosylation locations based on the derived amino acid sequences.The homology of coding amino acid between this strain and American 00-2601and domestic HL strain was 91.6% and 91.1%respectively,it showed that they had common ancestor.

Key concepts: Canine distemper, GenBank, Cloning (programming), Biology, Gene, Virology, Homology (biology), Polymerase chain reaction

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and Sequence Analysis of Fusion Protein Gene of Canine Distemper Virus Isolated from Guizhou — Research Paper | ScholarLens