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2008•China Animal Husbandry & Veterinary MedicineRequires access

The Cloning and Sequence Analysis of M Protein Coding Gene of SH Strain of Canine Distemper Virus

Siqing Zhang

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Abstract

According to the reported gene sequence of M protein of canine distemper virus(CDV) in GenBank,a special pair of primers were designed to RT-PCR amplify M protein gene by using the total RNA of CDV SH stain as a template.The results showed that the homology of gene sequence between CDV SH strain and A75/17,Onderstepoort stain and other seven strain were above 95%;the homology of coding amino acid between CDV SH strain and the others were above 97%.From this information,it can be predicted that M protein has highly conservative property.

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What this paper is about

According to the reported gene sequence of M protein of canine distemper virus(CDV) in GenBank,a special pair of primers were designed to RT-PCR amplify M protein gene by using the total RNA of CDV SH stain as a template.The results showed that the homology of gene sequence between CDV SH strain and A75/17,Onderstepoort stain and other seven strain were above 95%;the homology of coding amino acid between CDV SH strain and the others were above 97%.From this information,it can be predicted that M protein has highly conservative property.

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Available abstract

According to the reported gene sequence of M protein of canine distemper virus(CDV) in GenBank,a special pair of primers were designed to RT-PCR amplify M protein gene by using the total RNA of CDV SH stain as a template.The results showed that the homology of gene sequence between CDV SH strain and A75/17,Onderstepoort stain and other seven strain were above 95%;the homology of coding amino acid between CDV SH strain and the others were above 97%.From this information,it can be predicted that M protein has highly conservative property.

Key concepts: Canine distemper, GenBank, Homology (biology), Virology, Gene, Biology, Virus, Molecular biology

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The Cloning and Sequence Analysis of M Protein Coding Gene of SH Strain of Canine Distemper Virus — Research Paper | ScholarLens