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Sequence Analysis of Nucleocaspid Protein Gene of Vaccine Strain of Canine Distemper Virus

Feng Wang

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Abstract

Two pairs of PCR primers were designed according to the Nucleocaspid protein gene sequences of canine distemper virus(CDV) in GenBank.A 1632 bp fragment including Nucleocaspid(N) protein gene was amplified by RT-PCR with RNA template of CDV3.The PCR product was cloned and then sequenced.The homology between the N genes of CDV3 and vaccine strain Onderstepoort and 12 isolated strains in GenBank database was analyzed.The result showed that the homology of the nucleotide sequence between the CDV3 and vaccine strains Onderstepoort is 97.1%,and 92.8%～94.0% with other isolates.There is 93.6% homology between CDV3 and TN in China.However,96.6%～97.3% is of amino acid sequence.Then the result showed our vaccine stain CDV3 is closer in amino acid level than in nucleotide level with other strains.

About this research paper

What this paper is about

Two pairs of PCR primers were designed according to the Nucleocaspid protein gene sequences of canine distemper virus(CDV) in GenBank.A 1632 bp fragment including Nucleocaspid(N) protein gene was amplified by RT-PCR with RNA template of CDV3.The PCR product was cloned and then sequenced.The homology between the N genes of CDV3 and vaccine strain Onderstepoort and 12 isolated strains in GenBank database was analyzed.The result showed that the homology of the nucleotide sequence between the CDV3 and vaccine strains Onderstepoort is 97.1%,and 92.8%～94.0% with other isolates.There is 93.6% homology between CDV3 and TN in China.However,96.6%～97.3% is of amino acid sequence.Then the result showed our vaccine stain CDV3 is closer in amino acid level than in nucleotide level with other strains.

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Available abstract

Two pairs of PCR primers were designed according to the Nucleocaspid protein gene sequences of canine distemper virus(CDV) in GenBank.A 1632 bp fragment including Nucleocaspid(N) protein gene was amplified by RT-PCR with RNA template of CDV3.The PCR product was cloned and then sequenced.The homology between the N genes of CDV3 and vaccine strain Onderstepoort and 12 isolated strains in GenBank database was analyzed.The result showed that the homology of the nucleotide sequence between the CDV3 and vaccine strains Onderstepoort is 97.1%,and 92.8%～94.0% with other isolates.There is 93.6% homology between CDV3 and TN in China.However,96.6%～97.3% is of amino acid sequence.Then the result showed our vaccine stain CDV3 is closer in amino acid level than in nucleotide level with other strains.

Key concepts: GenBank, Canine distemper, Homology (biology), Biology, Gene, Virology, Virus, Nucleic acid sequence

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Sequence Analysis of Nucleocaspid Protein Gene of Vaccine Strain of Canine Distemper Virus — Research Paper | ScholarLens