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2006•Heilongjiang xumu shouyiRequires access

Effect of induce concentration, time and temperature of IPTG on the expression of the GST-GnRH/TRS gene

Jin Yuan-chang

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Abstract

The object is recombinant GnRH2'S gene and transporter gene with fusion expression vector pGEX-6p-1, transformed into the BL21(ED3) plysS prokaryotic expression system, induced and expressed by IPTG, analyzed the effect of different concentration 、time and temperature of IPTG on the expression of the GST-GnRH/TRS gene, confirmed the best induced condition of IPTG. SDS-PAGE analysis showed that GST-GnRH/TRS expression quantity was the most when temperature of IPTG was 30 ℃ and concentration of IPTG was 0.2 mmol/L. The amount of the goal protein was evaluated by densitometric scanning. It indicated that the product of the GST-GnRH/TRS gene was 33% of total bacterial protein of BL21(ED3).

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What this paper is about

The object is recombinant GnRH2'S gene and transporter gene with fusion expression vector pGEX-6p-1, transformed into the BL21(ED3) plysS prokaryotic expression system, induced and expressed by IPTG, analyzed the effect of different concentration 、time and temperature of IPTG on the expression of the GST-GnRH/TRS gene, confirmed the best induced condition of IPTG. SDS-PAGE analysis showed that GST-GnRH/TRS expression quantity was the most when temperature of IPTG was 30 ℃ and concentration of IPTG was 0.2 mmol/L. The amount of the goal protein was evaluated by densitometric scanning. It indicated that the product of the GST-GnRH/TRS gene was 33% of total bacterial protein of BL21(ED3).

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Available abstract

The object is recombinant GnRH2'S gene and transporter gene with fusion expression vector pGEX-6p-1, transformed into the BL21(ED3) plysS prokaryotic expression system, induced and expressed by IPTG, analyzed the effect of different concentration 、time and temperature of IPTG on the expression of the GST-GnRH/TRS gene, confirmed the best induced condition of IPTG. SDS-PAGE analysis showed that GST-GnRH/TRS expression quantity was the most when temperature of IPTG was 30 ℃ and concentration of IPTG was 0.2 mmol/L. The amount of the goal protein was evaluated by densitometric scanning. It indicated that the product of the GST-GnRH/TRS gene was 33% of total bacterial protein of BL21(ED3).

Key concepts: lac operon, Gene, Recombinant DNA, Fusion protein, Molecular biology, Gene expression, Biology, Chemistry

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Effect of induce concentration, time and temperature of IPTG on the expression of the GST-GnRH/TRS gene — Research Paper | ScholarLens