Effect of induced concentration of IPTG on the expression of recombinant Listeriolysin O

The recombinant of pGEX-6P-hly was transformed into E.coli BL-21,induced and expressed by IPTG,to analyze the effect of different concentration of IPTG on the expression of recombinant Listeriolysin O,and to confirm the best induced concentration of IPTG.Experimental results indicated that under shake incubator,LB medium and inducing 2.4 hours with 37 ℃,hly expression quantity was the most when ultima concentration of IPTG was 0.6 mmol·L-1.Western-blot analysis showed that recombinant fusion protein could react with a specific anti-serum.This provided dependable experimental accordance for industry production of Listeriolysin O when IPTG was inducer.