Effect of Induced Concentration and Time of IPTG on the Expression of VP1 Protein of FMDV Type Asia I

The VP1 gene of FMDV type Asia I was inserted into the prokaryotic expression vector pET-28a,and then transformed into BL21 cells.The recombinant plasimd was induced with different concentration and time of IPTG to express the VP1 protein.The better expression condition was confirmed by analyzing the effect of different concentration and time of IPTG on the expression product.The experimental result indicated that the expression product had not been increased with adding of the concentration of IPTG in definite scope,but the expression output had been increased with the extension of time during the period of logarithmic growth of bacteria.The maximal quantity of expression of VP1 protein was confirmed when the concentration of IPTG was 3 mmol/L and the induced time was 9 hours.