Cloning and Sequence Analysis of FADX Gene from Vernicia fordii

α-eleostearic acid synthetase is the important enzyme for converting linoleic acid into α-eleostearic acid.Total RNA was successfully extracted from growing Vernicia fordii seeds with improved TRIzoL method.cDNA of FADX was cloned by RT-PCR.Bioinformatic analysis showed that the cDNA had length of 1221bp an ORF(14～1174 bp)which encoded a polypeptide of 386 amino acids,and contained a typical fatty acid dehydrogenation structural domain.In order to introduce the gene into plant,the cDNA was ligated to the pBI121 in the antisense orientation after digested with the enzyme BamHI and SacI.The new vector named pBI121fadx was still transformed to leaf of tobacco via Agrobacterium tumefaciens system.