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2011•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Oleanolic Acid in Suosuo-grape Granule by HPLC Method

Xuan Zhang

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Abstract

Objective To estabilsh an HPLC method for oleanolic acid in Suosuo-grape Granule.Methods The separation was carried out on a C18 column,with mobile phase of methanol-water(85:15).The flow rate was set at 1.0ml·min-1 and the detection wavelength was at 220nm.Results The calibration curve of oleanolic acid was in good linearity within the range of 3.2~22.4μg(r=0.999 5),and the average recovery rate was 101.2%.Conclusion The developed method is sensitive,accurate,and it can be used for the determination of oleanolic acid in TCM preparation and the quality control of Suosuo-grape Granule.

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What this paper is about

Objective To estabilsh an HPLC method for oleanolic acid in Suosuo-grape Granule.Methods The separation was carried out on a C18 column,with mobile phase of methanol-water(85:15).The flow rate was set at 1.0ml·min-1 and the detection wavelength was at 220nm.Results The calibration curve of oleanolic acid was in good linearity within the range of 3.2~22.4μg(r=0.999 5),and the average recovery rate was 101.2%.Conclusion The developed method is sensitive,accurate,and it can be used for the determination of oleanolic acid in TCM preparation and the quality control of Suosuo-grape Granule.

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Available abstract

Objective To estabilsh an HPLC method for oleanolic acid in Suosuo-grape Granule.Methods The separation was carried out on a C18 column,with mobile phase of methanol-water(85:15).The flow rate was set at 1.0ml·min-1 and the detection wavelength was at 220nm.Results The calibration curve of oleanolic acid was in good linearity within the range of 3.2~22.4μg(r=0.999 5),and the average recovery rate was 101.2%.Conclusion The developed method is sensitive,accurate,and it can be used for the determination of oleanolic acid in TCM preparation and the quality control of Suosuo-grape Granule.

Key concepts: Oleanolic acid, Chromatography, Granule (geology), High-performance liquid chromatography, Calibration curve, Chemistry, Content determination, Methanol

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