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2011•Research and Practice on Chinese MedicinesRequires access

Determination of Paeoniflorin in Danggui Yangxue Pill by HPLC

XU Qing-hui

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Abstract

Objective To establish the method for quantitative determination of Paeoniflorin in Danggui Yangxue Pill.Methods HPLC was used in this study.Kromasil C18(4.6 mm×250 mm,5μm)colunm was used and the mobile phase was acetonitrile-0.1% phosphoric acid(14:86).The detecition wavelength was set at 230nm.The flow rate was 1.0 mL/min.Results The linear range of Paeoniflorin were 12.68~126.8 μg/mL(r=0.999 9).The average recovery rate and RSD were 99.32%and 0.38%.Conclusion The method is simple,accurate,reliable,and can effectively control the quality of Danggui Yangxue Pill.

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What this paper is about

Objective To establish the method for quantitative determination of Paeoniflorin in Danggui Yangxue Pill.Methods HPLC was used in this study.Kromasil C18(4.6 mm×250 mm,5μm)colunm was used and the mobile phase was acetonitrile-0.1% phosphoric acid(14:86).The detecition wavelength was set at 230nm.The flow rate was 1.0 mL/min.Results The linear range of Paeoniflorin were 12.68~126.8 μg/mL(r=0.999 9).The average recovery rate and RSD were 99.32%and 0.38%.Conclusion The method is simple,accurate,reliable,and can effectively control the quality of Danggui Yangxue Pill.

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Available abstract

Objective To establish the method for quantitative determination of Paeoniflorin in Danggui Yangxue Pill.Methods HPLC was used in this study.Kromasil C18(4.6 mm×250 mm,5μm)colunm was used and the mobile phase was acetonitrile-0.1% phosphoric acid(14:86).The detecition wavelength was set at 230nm.The flow rate was 1.0 mL/min.Results The linear range of Paeoniflorin were 12.68~126.8 μg/mL(r=0.999 9).The average recovery rate and RSD were 99.32%and 0.38%.Conclusion The method is simple,accurate,reliable,and can effectively control the quality of Danggui Yangxue Pill.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Phosphoric acid, Linear range, Chemistry, Medicine, Detection limit

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