The Effect of NM-3 on Cells Proliferation and Apoptosis of the Human Gastric Cancer Cell SGC-7901 in vitro

Objective: To study the action of NM3, which is a kind of angiogenesis inhibitor, on the growth and apoptosis of human gastric carcinoma cell line SGC-7901 and discuss its action mechanism. Methods:Human gastric carcinoma cell line SGC-7901 was treated with NM-3, Carboplatin and NS Cell proliferation was evaluated with MTT assay, Distributions of cell cycle and apoptosis rate were determined with flow cytometry, cell apoptosis was confirmed by morphology. Results:The inhibition effect of NM-3 was not apparent on the proliferation of SGC-7901 compared with controls, the apoptosis rate was not statistically different (P0. 05). In cell cycle analysis, NM-3 could not increase the proportion of cells in the G0/G1 phase which was an indication of early apoptosis. Conclusion:NM-3 could not induce the apoptosis of human gastric cancer cell SGC-7901 in vitro, it enhanced the sensitivity of Carboplatin on human gasric cancer cell SGC-7901 in vitro. It had no direct effect on proliferation of gastric carcinoma cells. The anticancer mechanism of NM-3 in animal model was mainly related to its antiangiogenesis action.