Effect of 2-methoxyestradiol on Proliferation and Apoptosis of SGC-7901 Cells

Objective To explore the effects of 2-methoxyestradiol(2-ME) on proliferation,apoptosis and cell cycle of SGC-7901 cells in vitro. Methods SGC-7901 cells were treated with 2-ME of different concentrations and time in vitro,morphological changes of apoptotic cells were observed under microscope;cell proliferation inhibition was detected by MTT colorimetric assay;cell cycle arrest and apoptosis rate were measured by flow cytometry(FCM). Results Exposure to 2-ME had a strong antiproliferative effect on human gastric cancer cells and caused an increase in the population of apoptotic cells.There was significant difference between 2-ME group and control group(P0.05).A significant number of cells were blocked in the G2/M phases of the cell cycle,Concurrently,the population of cells in the G0/G1 and S phases decreased in SGC-7901 cell lines,detected by flow cytometry.There was significant difference between 2-ME group and control group(P0.05). Conclusion 2-ME inhibits the growth of human gastric cancer cells and induces apoptosis.