Experimental Research on Anti-inflammatory and Analgesic Effect of Optimized Huoxue Lishui Fang

Objective :To investigate the effects induced by Huoxue Lishui Fang on Non-compressive model study of experimental lumbar nucleus pulposus prolapse breeding nerve root pain in rats by transplantation of autologous nucleus pulposus.Methods: 36 mice were chosen.The mice were divided into the control group,the Chinese medicines group and the aspirin group.NS,Decoction of Chinese medicine and aspirin were given separately once a day.After continuous medication for 5 days,writhing number of the mice induced by acetic acid was tested.Another 36 mice were taken and swelling degree of mouse ear induced by xylene was tested in the same way(groups,drug delivery).40 SD(Sprague-dawley) rats were chosen.They were randomly divided into the control group,model group,the Chinese medicines group and the aspirin group.L5 nerve root pain model induced by transplantation of autologous nucleus pulposus in rats was built.A week later,NS,Decoction of Chinese medicine and aspirin were given orally to groups separately once a day,with medication for 4 weeks in a row.1 day before the surgical operation,the 3rd day and from week 1 to week 5 after the surgical operation,test of rats' left hind mechanical stimulation pain threshold was made.Results: Optimized Huoxue Lishui Fang significantly reduced the writhing number of the mice induced by acetic acid,meanwhile alleviate swelling degree of mouse ear induced by xylene.It improved the rats’left hind mechanical stimulation pain threshold significantly.Conclusion: Optimized Huoxue Lishui Fang has significant anti-inflammatory and analgesic effect to Non-compressive model study of experimental lumbar nucleus pulposus prolapse breeding nerve root pain in rats by transplantation of autologous nucleus pulposus.