2016•Pain Clin JRequires access
Establishment of a new rat model of lumbar disc herniation using in minimally invasive interventional surgery
Chao Deng, Xianguang Wang, Huiying Li, Sitong Liu, Zhenhua Cai, Zhou Huacheng
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Abstract
Objective
To establish a new model of lumbar disc herniation for minimally invasive interventional surgery in rats.
Methods
Ninety SD rats weighted 200-250 g were randomly divided into sciatic nerve compression group (group A) , classical model group (group B) and sham operation group (group C) , 30 rats in each group. Autologous nucleus pulposus (NP) obtained from the coccygeal intervertebral discs was implanted onto the right sciatic nerve, and ligated the sciatic nerve in group A. Nucleus pulposus was applied onto the right L5 nerve root and ligated it in group B. The sciatic nerve was exposed only in group C, without transplantation and ligation. The mechanical pain threshold and thermal pain threshold were tested respectively, and the temperature of lower limbs of rats were measured by infrared thermography at 1 day before surgery and 1, 3, 5, 7, 14 days after surgery in all groups. 15 rats selected randomly were sacrificed on the seventh and fourteenth day respectively after surgery in each group and IL-8 and TNF-α expression level of the L5 dorsal root ganglion and sciatic nerve tissues were tested by immunohistochemical method.
Results
Compared with group C, the mechanical pain threshold and thermal pain threshold were lower in group A and B at 3, 5, 7, 14 days after surgery. At the same time, the average temperature of lower limbs reduced significantly. The positive cells of IL-8 and TNF-α in the L5 dorsal root ganglion and sciatic nerve tissues increased at 7 days after surgery, reduced 14 days after surgery, but were still higher than those in group C (P 0.05) between group A and B.
Conclusion
New rat model as the classical model can imitate exactly the cause of lumbar disc herniation with less damage and easier operation compared with classical model. Meanwhile the new model provides good conditions for surgical intervention than classical model.
Key words:
Models, animal; Rats; Intervertebral disk displacement; Inflammatory factors