An Investigation of Ultra Structure of Transplanted Nucleus Pulposus and Nerve Root of Transplantation of Autologous Nucleus Pulposus in Rats Intervened by Huoxue Lishui Fang

Objective:To design Non-compressive model study of experimental lumbar nucleus pulposus prolapse breeding nerve root pain in rats by transplantation of autologous nucleus pulposus,using aspirin as the positive control drug,to investigate the effects induced by Huoxue Lishui Fang on ultra structure of transplanted nucleus pulposus and nerve root through TEM.Methods:40 SD(Sprague-dawley) rats were chosed.The rats were randomly divided into the control group,model group,the Chinese medicines group and the aspirin group.To design L5 nerve root pain model induced by transplantation of autologous nucleus pulposus in rats.A week later,1.82mL NS,1.82mL NS,1.82mL Decoction of Chinese medicine and 1.82mL aspirin(0.1g·kg-1·d-1) were given separately once a day.Medication for 4 weeks in a row.On the 3rd day and the 7th day after medecine was given,test of rats′ left hind mechanical stimulation pain threshold was made.A month later,the left side of the transplanted nucleus and L5 root Histopathology were taken out for electron microscopy.Results:3and 7 days after the surgical operation,The left hind mechanical stimulation of the model group,the group of western medicine and the Chinese medicine group were significantly lower than the pre-operative pain threshold.Ultra structure showed the ultra structure of the control group is normal,the model group was significantly abnormal,Western medicine group and Chinese medicine group were significantly better than the model group,the Chinese medicine group was slightly better than the western medicine group.Conclusion:Optimized Huoxue Lishui Fang helped to relieve pathological damage of nucleus pulposus and nerve root.