Distribution and antimicrobial resistance of major clinical pathogens isolated from 2008 to 2010

Objective To investigate the distribution and antimicrobial resistance of pathogens from patients in a hospital,and provide basis for rational use of antimicrobial agents.Methods Data about bacterial strains isolated from inpatients and outpatients in a hospital from January 2008 to December 2010 were collected,distribution and antimicrobial resistance of pathogens were analyzed.Results A total of 7 008 bacterial strains were isolated,3 961(56.52%) isolates were gram-negative bacteria,1 582(22.57%) were gram-positive bacteria,and 1 465(20.91%) were fungi;the top three pathogens were Candida albicans(1 015 isolates,15.00%),Pseudomonas aeruginosa(906 isolates,12.93%),Escherichia coli(874 isolates,12.47%).Methicillin-resistant Staphylococcus aureus(MRSA) accounted for 85.07%(678/797) of all Staphylococcus aureus,methicillin-resistant coagulase-negative Staphylococcus(MRCNS) accounted for 73.17%(150/205) of all CNS;the isolation rate of extended-spectrum β-lactamase(ESBLS)-producing Escherichia coli and Klebsiella pneumoniae was 64.19%(561/874) and 46.31%(301/650) respectively.Staphylococcus aureus isolates were sensitive to vancomycin and teicoplanin,the resistant rate to sulfamethoxazole/trimethoprim was 68.42%-74.51%,the resistant rate to the other agents were all 30%;isolates of CNS were sensitive to vancomycin and teicoplanin;Escherichia coli and Klebsiella pneumoniae had highest sensitive rate to imipenem and meropenem(95.52%-100.00%),the resistant rate of Escherichia coli to cefotaxime,ceftriaxone,cefoperazone,ciprofloxacin,and levofloxacin were all 30%.Pseudomonas aeruginosa had relative higher resistant rate to ceftazidime(60.31%-85.83%) and meropenem(59.38%-73.23%).Conclusion Antimicrobial resistance of major pathogens isolated from patients in this hospital were generally high.It should pay more attention to the monitoring of multi-drug resistant strains,antimicrobial agents should be used rationally.