2010•Food Science and Technology InternationalRequires access
Development of sandwich-antibody enzyme linked immunosorbent assay for the rapid detection of casein in milk
Fang Li
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Abstract
Casein was used as the antigen to immune the New Zealand rabbits and BALB/C mice respectively in the experiment.The anti-casein polyclonal antibodies and monoclonal antibodies were prepared.The monoclonal antibody was used as the coating antibody,and HRP-polyclonal antibody was retained.The sandwich ELISA to detect the casein of milk was developed successfully,which had a standard curve in the 6～ 1666 ng/mL range of good linearity with r2=0.9949.The detection limit of the method was 7 ng/mL.The method could be use as a theory and technological base of casein detection of milk adulteration and food allergen test.