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Establishment of indirect ELISA of bovine β-casein

Jing Meng

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Abstract

The polyclonal antibody was prepared by immunizing New Zealand rabbit with bovine β-casein which was extracted from casein. The collected sera was then purified through precipitating using amine sulphate in order to prepare the polyclonal antibody against bovine β-casein which could be used to detection of bovine β-casein according to the indirect ELISA.

About this research paper

What this paper is about

The polyclonal antibody was prepared by immunizing New Zealand rabbit with bovine β-casein which was extracted from casein. The collected sera was then purified through precipitating using amine sulphate in order to prepare the polyclonal antibody against bovine β-casein which could be used to detection of bovine β-casein according to the indirect ELISA.

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Available abstract

The polyclonal antibody was prepared by immunizing New Zealand rabbit with bovine β-casein which was extracted from casein. The collected sera was then purified through precipitating using amine sulphate in order to prepare the polyclonal antibody against bovine β-casein which could be used to detection of bovine β-casein according to the indirect ELISA.

Key concepts: Polyclonal antibodies, Casein, Bovine milk, Chemistry, Antibody, Chromatography, Bovine serum albumin, Food science

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