2014•Journal of Guangdong Industry Technical CollegeRequires access
Detection of α-Lactalbumin in Hot-processed Food by Sandwich-antibody Enzyme Linked Immunosorbent Assay
Zhang Tin
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Abstract
The aim of the research was to develop sandwich-antibody enzyme linked immunosorbent assay( ELISA)for α-lactalbumin in food products. Hot-processed α-lactalbumin was as antigen. In order to detect α-lactalbumin,the antibodies against α-lactalbumin were prepared. The sandwich-antibody ELISA method was established which used monoclonal antibody for the coating antibody and enzyme labeled polyclonal antibody for second antibody. The IC50 of standard curve was 360 ng /mL. The sensitivity of the method was 10 ng /mL. Urea could improve the recoveries of α-lactalbumin fortified which was between 76% ~ 89%. The RSD was less than 15%. The method could recognize the heat processing milk products,which could avoid misjudgement in analysis for heat processing products.