Determination of Magnolol and Honokiol in Bao Ying Dan by HPLC
Zou Yu-fan
Abstract
Zou Yu-fan
Abstract
Objective To establish a HPLC method for the determination of magnolol and honokiol in Bao Ying Dan.Methods A Kromasil C18 column was employed with the mobile phase consisting of methanol-water(78∶ 22) and the determination wavelength was 294nm.Results The linear range of magnolol was 0.1074~0.537μg(r = 0.9999).The linear range of honokiol was 0.0585~0.2925ug(r = 0.9997).The average recovery of magnolol was 99.52 % with RSD of 1.97%.The average recovery of honokiol was 97.68 % with RSD of 1.48%.Conclusion This method is accurate,reliable and reproducible.It can be used for the assaying of magnolol and honokiol in Bao Ying Dan.
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Objective To establish a HPLC method for the determination of magnolol and honokiol in Bao Ying Dan.Methods A Kromasil C18 column was employed with the mobile phase consisting of methanol-water(78∶ 22) and the determination wavelength was 294nm.Results The linear range of magnolol was 0.1074~0.537μg(r = 0.9999).The linear range of honokiol was 0.0585~0.2925ug(r = 0.9997).The average recovery of magnolol was 99.52 % with RSD of 1.97%.The average recovery of honokiol was 97.68 % with RSD of 1.48%.Conclusion This method is accurate,reliable and reproducible.It can be used for the assaying of magnolol and honokiol in Bao Ying Dan.
Key concepts: Magnolol, Honokiol, Chromatography, Chemistry, Linear range, High-performance liquid chromatography, Linear relationship, Detection limit