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2010•Ningxia Medical JournalRequires access

Determination of magnolol and honokiol in Shuganjianwei Wan by HPLC

Lei Chen

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Abstract

Objective To establish HPLC method for the content determination of magnolol and honokiol in Shuganjianwei Wan.Methods A spherisorb C18 column(4.6×250mm,5vm) was used as the analytical column and acetonitrile-water(62.5:37.5) as the mobile phase.The detection wavelength was 295nm.Results The calibration curves of magnolol and honokiol were linear over the range 0.32-1.62μg and 0.42-2.10μg(r=0.9999,0.9998) respectively.The average recovery of the method was 99.1%.Conclusion The method is rapid and accurate,and the results are reliable.

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What this paper is about

Objective To establish HPLC method for the content determination of magnolol and honokiol in Shuganjianwei Wan.Methods A spherisorb C18 column(4.6×250mm,5vm) was used as the analytical column and acetonitrile-water(62.5:37.5) as the mobile phase.The detection wavelength was 295nm.Results The calibration curves of magnolol and honokiol were linear over the range 0.32-1.62μg and 0.42-2.10μg(r=0.9999,0.9998) respectively.The average recovery of the method was 99.1%.Conclusion The method is rapid and accurate,and the results are reliable.

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Available abstract

Objective To establish HPLC method for the content determination of magnolol and honokiol in Shuganjianwei Wan.Methods A spherisorb C18 column(4.6×250mm,5vm) was used as the analytical column and acetonitrile-water(62.5:37.5) as the mobile phase.The detection wavelength was 295nm.Results The calibration curves of magnolol and honokiol were linear over the range 0.32-1.62μg and 0.42-2.10μg(r=0.9999,0.9998) respectively.The average recovery of the method was 99.1%.Conclusion The method is rapid and accurate,and the results are reliable.

Key concepts: Magnolol, Honokiol, Medicine, Calibration curve, Chromatography, High-performance liquid chromatography, Linear range, Detection limit

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