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2011•Central South PharmacyRequires access

HPLC determination of magnolol and honoliol in Shugan Kuaiwei Wan

Xiang Fei

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Abstract

Objective To establish an HPLC method to determine the content of magnolol and honokiol in Shugan Kuaiwei Wan. Methods HPLC was used to detect magnolol and honokiol chromatograph on Dikma Diamonsil C18(250 mm×4.6 mm,5 μm).The mobile phase consisted of methanol-water-glacialaceticacid(82∶18∶0.25),the dectected wavelength was 294 nm,and the column temperature was 35 ℃ at 1.0 mL·min-1. Results The magnolol linearity was 0.10-1.25 μg(r=0.999 9),and the average recovery was 98.4% with RSD=1.2%.The honokiol linearity was 0.091 70-1.14 μg(r=0.999 3),and the average recovery was 98.6% with RSD=1.3%. Conclusion The mothed is simple,accurate and reproducible.

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What this paper is about

Objective To establish an HPLC method to determine the content of magnolol and honokiol in Shugan Kuaiwei Wan. Methods HPLC was used to detect magnolol and honokiol chromatograph on Dikma Diamonsil C18(250 mm×4.6 mm,5 μm).The mobile phase consisted of methanol-water-glacialaceticacid(82∶18∶0.25),the dectected wavelength was 294 nm,and the column temperature was 35 ℃ at 1.0 mL·min-1. Results The magnolol linearity was 0.10-1.25 μg(r=0.999 9),and the average recovery was 98.4% with RSD=1.2%.The honokiol linearity was 0.091 70-1.14 μg(r=0.999 3),and the average recovery was 98.6% with RSD=1.3%. Conclusion The mothed is simple,accurate and reproducible.

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Available abstract

Objective To establish an HPLC method to determine the content of magnolol and honokiol in Shugan Kuaiwei Wan. Methods HPLC was used to detect magnolol and honokiol chromatograph on Dikma Diamonsil C18(250 mm×4.6 mm,5 μm).The mobile phase consisted of methanol-water-glacialaceticacid(82∶18∶0.25),the dectected wavelength was 294 nm,and the column temperature was 35 ℃ at 1.0 mL·min-1. Results The magnolol linearity was 0.10-1.25 μg(r=0.999 9),and the average recovery was 98.4% with RSD=1.2%.The honokiol linearity was 0.091 70-1.14 μg(r=0.999 3),and the average recovery was 98.6% with RSD=1.3%. Conclusion The mothed is simple,accurate and reproducible.

Key concepts: Magnolol, Honokiol, Chromatography, High-performance liquid chromatography, Chemistry, Content determination

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