Cloning and Sequencing of Full Vp4 Gene of Group A Porcine Rotavirus

The antigenic determinants of Vp4 gene of porcine group A Rotavirus were amplified from cell infected with rotavirus by the reverse transcription-polymerase chain reaction(RT-PCR).The length of full Vp4 gene was 2 331 bp.The products of RT-PCR were ligated with plasmid pGEM-Teasy and transformed to JM109.By the analysis of restriction endonuclease cutting,PCR and sequencing,the recombinant plasmids pGEM-T-Vp4 was constructed.The results of nucleotide and amino acid sequences analysis showed that the experimental rotavirus Vp4 gene has over 99% similarity with the rotavirus Vp4 genes of America strain and Heilongjiang strain,which has a positive open reading frame.