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2003•Zhongguo yufang shouyi xuebaoRequires access

Cloning and Sequencing of Rotavirus VP4、VP7

Peng Wang

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Abstract

The antigenic determinants of Rotavirus VP4 and VP7 genes were amplified from cell infected with rotavirus by reverse transcription_polymerase chain reaction(RT_PCR),the length of target genes was 81bp and 91bp.The products of RT_PCR were ligated with plasmid pGEM_T and transformated to E.coli DH5α.By the analysis of restriction endonuclease and PCR,the fragments of VP4 and VP7 were cloned and the recombinant plasmids PT_V4 and PT_V7 were constructed.The results of nucleotide sequencing showed that the inserted genes had positive reading frame.

About this research paper

What this paper is about

The antigenic determinants of Rotavirus VP4 and VP7 genes were amplified from cell infected with rotavirus by reverse transcription_polymerase chain reaction(RT_PCR),the length of target genes was 81bp and 91bp.The products of RT_PCR were ligated with plasmid pGEM_T and transformated to E.coli DH5α.By the analysis of restriction endonuclease and PCR,the fragments of VP4 and VP7 were cloned and the recombinant plasmids PT_V4 and PT_V7 were constructed.The results of nucleotide sequencing showed that the inserted genes had positive reading frame.

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Available abstract

The antigenic determinants of Rotavirus VP4 and VP7 genes were amplified from cell infected with rotavirus by reverse transcription_polymerase chain reaction(RT_PCR),the length of target genes was 81bp and 91bp.The products of RT_PCR were ligated with plasmid pGEM_T and transformated to E.coli DH5α.By the analysis of restriction endonuclease and PCR,the fragments of VP4 and VP7 were cloned and the recombinant plasmids PT_V4 and PT_V7 were constructed.The results of nucleotide sequencing showed that the inserted genes had positive reading frame.

Key concepts: Biology, Rotavirus, Restriction enzyme, Plasmid, Gene, Virology, Open reading frame, Cloning (programming)

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