ScholarLens Back to search
2004•Zhongguo yufang shouyi xuebaoRequires access

Cloning of VP4 gene from porcine rotavirus JL94 isolate and homologous comparison with reference strains

Yijing Li

Open publisher page 0 citations

Abstract

A pair of primers according to the published cDNA sequence of porcine rotavirus VP4 gene were designed, which were used in cloning and sequence analysis of VP4 gene from porcine rotavirus JL94 isolated in China. The full length gene encoding outer capid protein VP4 was amplified from the native JL94 isolate of porcine rotavirus by reverse transcription polymerase chain reaction(RTPCR). The cDNA of VP4 gene was inserted into pMD-18T vector and identified by sequencing. The result of its homology comparison with CRW-8 strain and BEN-307 strain is 96.98 % and 98.05 % respectively.

About this research paper

What this paper is about

A pair of primers according to the published cDNA sequence of porcine rotavirus VP4 gene were designed, which were used in cloning and sequence analysis of VP4 gene from porcine rotavirus JL94 isolated in China. The full length gene encoding outer capid protein VP4 was amplified from the native JL94 isolate of porcine rotavirus by reverse transcription polymerase chain reaction(RTPCR). The cDNA of VP4 gene was inserted into pMD-18T vector and identified by sequencing. The result of its homology comparison with CRW-8 strain and BEN-307 strain is 96.98 % and 98.05 % respectively.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A pair of primers according to the published cDNA sequence of porcine rotavirus VP4 gene were designed, which were used in cloning and sequence analysis of VP4 gene from porcine rotavirus JL94 isolated in China. The full length gene encoding outer capid protein VP4 was amplified from the native JL94 isolate of porcine rotavirus by reverse transcription polymerase chain reaction(RTPCR). The cDNA of VP4 gene was inserted into pMD-18T vector and identified by sequencing. The result of its homology comparison with CRW-8 strain and BEN-307 strain is 96.98 % and 98.05 % respectively.

Key concepts: Biology, Complementary DNA, Gene, Cloning (programming), Rotavirus, Homology (biology), Molecular biology, Sequence analysis

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning of VP4 gene from porcine rotavirus JL94 isolate and homologous comparison with reference strains — Research Paper | ScholarLens