Gene Cloning and Sequencing of Rotavirus VP7

The antigenic determinant of Rotavirus VP7 genes was amplified from cell infected by rotavirus by reverse transcription polymerase chain reaction(RT PCR). The lenth of target gene was 916 bp.The products of RT PCR were ligated with plasmid pGEM T and transformated to E.coli DH5α.By the analysis of restriction endonuclease and PCR ,the fragment of VP7 was cloned and the recombinant plasmid PT V7 was constructed.The result of nucleotid sequencing showed that the inserted gene had positive reading frame.