Optimization of the Culture Condition and Preliminary Separation of Aflatoxin Degrading Enzymes of the Strain Pleurotus ostreatus P1

In order to improve the degradation ability of the strain Pleurotus ostreatus P1,the culture condition of this strain was optimized. the best condition was as follows: culture temperature was 30 ℃,incubation speed was 200 r·min- 1,the pH of MSM medium was 6.0,and culturing the strain for 10 days. Under the Optimized condition,1000 ng AFB1 can be degraded to be 175. 7 ng by 790 μL Pleurotus ostreatus P1 fermentation and the degradation rate reached to82. 43%. Besides degrading AFB1,Pleurotus ostreatus P1 fermentation can also effectively degrade AFB2,AFG1 and AFG2. AFB1 degradation was made by multiple enzymes in Pleurotus ostreatus P1 fermentation with accumulative effects.The results will provide useful information for exploring aflatoxin-degradation eyzymes.