2007Anhui nongye kexueRequires access

Primary Discussion on the Mutagenesis Treatment of Pleurotus ostreatus Strain and its Application

LI Yong-wen

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Abstract

The study was conducted to discuss the mutagenesis treatment of Pleurotus ostreatus strain and its application.In the culture of P.ostreatus with potato glucose agar medium as basic medium and 4 formulations of bag cultivation(A,B,C and D),the ultraviolet radiation with irradiation time of 0.5,10 and 15 min was used for mutagenesis treatment on mycelium of P.ostreatus to select the good quality and high-yield strain.The strain of P.ostreatus was selected by ultraviolet radiation through mutagenesis treatment.Its mycelium showed the characters of rapid growth,white hyphae and haleness,etc.Compared with CK(solid medium with cotton seed skin),the mycelium from original strain and cultivated strain by the mutagenesis treatment could grow well ahead 10~15 days.The mycelium from liquid strain could grow full of the bag earlier of 10~15 days than that from solid strain and form mushroom earlier of 20~30 days than that from solid strain.The yield had some difference in different medium formulas.The formula A with 87% cotton seed skin and 10% bran or chaff as main components had highest yield with the biology efficiency of 98.7.Producing the strain by liquid culture and cultivatingg P.ostreatus by medium formula had advantages of short culture cycle,simple culture procedure,low cost,good quality,high yield and finished product rate.

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The study was conducted to discuss the mutagenesis treatment of Pleurotus ostreatus strain and its application.In the culture of P.ostreatus with potato glucose agar medium as basic medium and 4 formulations of bag cultivation(A,B,C and D),the ultraviolet radiation with irradiation time of 0.5,10 and 15 min was used for mutagenesis treatment on mycelium of P.ostreatus to select the good quality and high-yield strain.The strain of P.ostreatus was selected by ultraviolet radiation through mutagenesis treatment.Its mycelium showed the characters of rapid growth,white hyphae and haleness,etc.Compared with CK(solid medium with cotton seed skin),the mycelium from original strain and cultivated strain by the mutagenesis treatment could grow well ahead 10~15 days.The mycelium from liquid strain could grow full of the bag earlier of 10~15 days than that from solid strain and form mushroom earlier of 20~30 days than that from solid strain.The yield had some difference in different medium formulas.The formula A with 87% cotton seed skin and 10% bran or chaff as main components had highest yield with the biology efficiency of 98.7.Producing the strain by liquid culture and cultivatingg P.ostreatus by medium formula had advantages of short culture cycle,simple culture procedure,low cost,good quality,high yield and finished product rate.

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Available abstract

The study was conducted to discuss the mutagenesis treatment of Pleurotus ostreatus strain and its application.In the culture of P.ostreatus with potato glucose agar medium as basic medium and 4 formulations of bag cultivation(A,B,C and D),the ultraviolet radiation with irradiation time of 0.5,10 and 15 min was used for mutagenesis treatment on mycelium of P.ostreatus to select the good quality and high-yield strain.The strain of P.ostreatus was selected by ultraviolet radiation through mutagenesis treatment.Its mycelium showed the characters of rapid growth,white hyphae and haleness,etc.Compared with CK(solid medium with cotton seed skin),the mycelium from original strain and cultivated strain by the mutagenesis treatment could grow well ahead 10~15 days.The mycelium from liquid strain could grow full of the bag earlier of 10~15 days than that from solid strain and form mushroom earlier of 20~30 days than that from solid strain.The yield had some difference in different medium formulas.The formula A with 87% cotton seed skin and 10% bran or chaff as main components had highest yield with the biology efficiency of 98.7.Producing the strain by liquid culture and cultivatingg P.ostreatus by medium formula had advantages of short culture cycle,simple culture procedure,low cost,good quality,high yield and finished product rate.

Key concepts: Mycelium, Pleurotus ostreatus, Strain (injury), Food science, Yield (engineering), Mushroom, Horticulture, Bran

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