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2013•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Puerarin in Chaiyin Oral Solution by HPLC

Zhou Liang

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Abstract

Objective: To establish an HPLC method for determination of puerarin in Chaiyin oral solution.Method: The Agilent Eclipse XDB-C18(4.6 mm × 150 mm,5 μm) was used,the mobile phase consisted of methanol-water(25 ∶ 75);the flow rate was 1.0 mL.min-1;the detection wavelength was set at 250 nm;the column temperature was kept at 30 ℃.Result: The puerarin peak was well separated with other peaks,and a good linear correlation was observed within the range of 0.020 64-0.825 6 μg(r = 0.999 9).The average recovery was 100.44% with RSD 1.45%(n = 6).Conclusion: The method is rapid and simple,it can be used for the quality control of Chaiyin oral solution with good selectivity,sensitivity and repeatability.

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What this paper is about

Objective: To establish an HPLC method for determination of puerarin in Chaiyin oral solution.Method: The Agilent Eclipse XDB-C18(4.6 mm × 150 mm,5 μm) was used,the mobile phase consisted of methanol-water(25 ∶ 75);the flow rate was 1.0 mL.min-1;the detection wavelength was set at 250 nm;the column temperature was kept at 30 ℃.Result: The puerarin peak was well separated with other peaks,and a good linear correlation was observed within the range of 0.020 64-0.825 6 μg(r = 0.999 9).The average recovery was 100.44% with RSD 1.45%(n = 6).Conclusion: The method is rapid and simple,it can be used for the quality control of Chaiyin oral solution with good selectivity,sensitivity and repeatability.

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Available abstract

Objective: To establish an HPLC method for determination of puerarin in Chaiyin oral solution.Method: The Agilent Eclipse XDB-C18(4.6 mm × 150 mm,5 μm) was used,the mobile phase consisted of methanol-water(25 ∶ 75);the flow rate was 1.0 mL.min-1;the detection wavelength was set at 250 nm;the column temperature was kept at 30 ℃.Result: The puerarin peak was well separated with other peaks,and a good linear correlation was observed within the range of 0.020 64-0.825 6 μg(r = 0.999 9).The average recovery was 100.44% with RSD 1.45%(n = 6).Conclusion: The method is rapid and simple,it can be used for the quality control of Chaiyin oral solution with good selectivity,sensitivity and repeatability.

Key concepts: Puerarin, Repeatability, Chromatography, High-performance liquid chromatography, Linear range, Chemistry, Linear correlation, Linear relationship

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