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2014•Medical & Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Content Detection of Puerarin in Gegenjianghuang Oral Liquid by High Performance Liquid Chromatography

Xian Li

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Abstract

Objective To establish a method for content detection of Puerarin in Gegenjianghuang oral liquid.Methods The chromatographic column was Agilent Eclipse XDB-C18( 4. 6 mm × 150 mm,5 μm); the mobile phase consisted of methanol-water( 30∶ 70); the flow rate was 1. 0 ml /min; the detection wavelength was at 250 nm; the column temperature was at 30℃. Results The values of Puerarin within the range of 0. 02 064- 0. 4 128 g had good linear correlation with the values of peak area( r = 0. 9 998),the average recovery rate was 99. 29% with 1. 57% of relative standard deviation( RSD). Conclusion The high performance liquid chromatography( HPLC) method can be used for the quality control and evaluation of Gegenjianghuang oral liquid.

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Objective To establish a method for content detection of Puerarin in Gegenjianghuang oral liquid.Methods The chromatographic column was Agilent Eclipse XDB-C18( 4. 6 mm × 150 mm,5 μm); the mobile phase consisted of methanol-water( 30∶ 70); the flow rate was 1. 0 ml /min; the detection wavelength was at 250 nm; the column temperature was at 30℃. Results The values of Puerarin within the range of 0. 02 064- 0. 4 128 g had good linear correlation with the values of peak area( r = 0. 9 998),the average recovery rate was 99. 29% with 1. 57% of relative standard deviation( RSD). Conclusion The high performance liquid chromatography( HPLC) method can be used for the quality control and evaluation of Gegenjianghuang oral liquid.

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Available abstract

Objective To establish a method for content detection of Puerarin in Gegenjianghuang oral liquid.Methods The chromatographic column was Agilent Eclipse XDB-C18( 4. 6 mm × 150 mm,5 μm); the mobile phase consisted of methanol-water( 30∶ 70); the flow rate was 1. 0 ml /min; the detection wavelength was at 250 nm; the column temperature was at 30℃. Results The values of Puerarin within the range of 0. 02 064- 0. 4 128 g had good linear correlation with the values of peak area( r = 0. 9 998),the average recovery rate was 99. 29% with 1. 57% of relative standard deviation( RSD). Conclusion The high performance liquid chromatography( HPLC) method can be used for the quality control and evaluation of Gegenjianghuang oral liquid.

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Relative standard deviation, Chemistry, Volumetric flow rate, Linear correlation, Recovery rate

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