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Determination of Puerarin in Niuge Oral Liquid with RP-HPLC

Ye Dong

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Abstract

Objective:To set up a method for the determination of puerarin in niuge oral liquid with high perfomance liquid chromatography (HPLC). Methods:A Nova Pak C 18 chromatographic column(3.9 mm×150.0 mm, 4 μm) served as the stationary phase, while a mixture of methanol H 2O=25∶75( V/V ), as the mobile phase. The flow rate was 0.7 mL·min 1 and the wavelength for detection was 250 nm. Results:A good linear correlation was shown when the concentration of puerarin lay within a range of 0.404-2.020 μg, r =0.999 9. The average rate of recovery was 99.23%, RSD =1.29%; and the RSD value of precision for continuous sample mountings was 0.26%. Conclusion:Interference of other components could eliminated by this method, which was shown to be accurate, reliable, simple and convenient with good reprodu cibility.

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Objective:To set up a method for the determination of puerarin in niuge oral liquid with high perfomance liquid chromatography (HPLC). Methods:A Nova Pak C 18 chromatographic column(3.9 mm×150.0 mm, 4 μm) served as the stationary phase, while a mixture of methanol H 2O=25∶75( V/V ), as the mobile phase. The flow rate was 0.7 mL·min 1 and the wavelength for detection was 250 nm. Results:A good linear correlation was shown when the concentration of puerarin lay within a range of 0.404-2.020 μg, r =0.999 9. The average rate of recovery was 99.23%, RSD =1.29%; and the RSD value of precision for continuous sample mountings was 0.26%. Conclusion:Interference of other components could eliminated by this method, which was shown to be accurate, reliable, simple and convenient with good reprodu cibility.

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Available abstract

Objective:To set up a method for the determination of puerarin in niuge oral liquid with high perfomance liquid chromatography (HPLC). Methods:A Nova Pak C 18 chromatographic column(3.9 mm×150.0 mm, 4 μm) served as the stationary phase, while a mixture of methanol H 2O=25∶75( V/V ), as the mobile phase. The flow rate was 0.7 mL·min 1 and the wavelength for detection was 250 nm. Results:A good linear correlation was shown when the concentration of puerarin lay within a range of 0.404-2.020 μg, r =0.999 9. The average rate of recovery was 99.23%, RSD =1.29%; and the RSD value of precision for continuous sample mountings was 0.26%. Conclusion:Interference of other components could eliminated by this method, which was shown to be accurate, reliable, simple and convenient with good reprodu cibility.

Key concepts: Puerarin, Chromatography, Chemistry, High-performance liquid chromatography, Volumetric flow rate, Linear correlation, Linear range, Analytical Chemistry (journal)

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