Influence of sample hemolysis on the results and Countermeasures in clinical biochemistry

Objective To study the effect of hemolysis on biochemical test results.Methods Blood samples from 50 normal subjects for examination as the research object,using various biochemical tests of blood specimens of automatic biochemical analyzer in(including TP,TBIL,AST,DBIL,ALT,CK,γ-GT,ALB,LDH,TC,HDL,TG,LDL) measured variation value,and biochemical indicators of hemolysis and comparative analysis.Results The results were analyzed,compared with the normal specimens,hemolysis specimens of DBIL,ALB,TC,TG,HDL,TBIL,LDL values did not change significantly,the difference was not statistically significant(P0.05),while CK,TP,AST,LDH,ALT,γ-GT obviously increased,the difference was statistically signifcant(P0.05).Conclusion Clinical examination,hemolysis on serum AST,ALT,CK,TP,γ-GT,LDH and other biochemical indexes have significant interference on the determination results,in carries on the analysis,we must pay attention to whether hemolysis,and to take all feasible measures and methods,controlling the occurrence of hemolysis,necessary to blood,to ensure the accuracy of determination results.