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2009•Journal of Medical ForumRequires access

Effects of Rosiglitazone on Rat Kidney Fibroblasts Cultured in High Glucose

LI Hai-jian

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Abstract

Objective To investigate the protective effects of rosiglitazone on rat kidney fibroblasts(NRK) damaged by high glucose.Methods The NRK cells were cultured in vitro,and were divided into five groups: normal glucose group(NG,1000 mg/L D-glucose),high glucose group(HG,4500 mg/L D-glucose),HG+RGZ(5μmol/L),HG+RGZ(10μmol/L) and HG+RGZ(15μmol/L).The mRNA expressions of TGF-β1 and PAI-1 were measured using RT-PCR.Results Compared with NG group,mRNA expressions of TGF-β1 and PAI-1 increased significantly in HG group,while rosiglitazone inhibited those changes in dose-dependent manner.Conclusion Rosiglitazone can ameliorate the damage on the kidney fibroblasts induced by high glucose,thus have protecting effects on kidney.

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What this paper is about

Objective To investigate the protective effects of rosiglitazone on rat kidney fibroblasts(NRK) damaged by high glucose.Methods The NRK cells were cultured in vitro,and were divided into five groups: normal glucose group(NG,1000 mg/L D-glucose),high glucose group(HG,4500 mg/L D-glucose),HG+RGZ(5μmol/L),HG+RGZ(10μmol/L) and HG+RGZ(15μmol/L).The mRNA expressions of TGF-β1 and PAI-1 were measured using RT-PCR.Results Compared with NG group,mRNA expressions of TGF-β1 and PAI-1 increased significantly in HG group,while rosiglitazone inhibited those changes in dose-dependent manner.Conclusion Rosiglitazone can ameliorate the damage on the kidney fibroblasts induced by high glucose,thus have protecting effects on kidney.

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Available abstract

Objective To investigate the protective effects of rosiglitazone on rat kidney fibroblasts(NRK) damaged by high glucose.Methods The NRK cells were cultured in vitro,and were divided into five groups: normal glucose group(NG,1000 mg/L D-glucose),high glucose group(HG,4500 mg/L D-glucose),HG+RGZ(5μmol/L),HG+RGZ(10μmol/L) and HG+RGZ(15μmol/L).The mRNA expressions of TGF-β1 and PAI-1 were measured using RT-PCR.Results Compared with NG group,mRNA expressions of TGF-β1 and PAI-1 increased significantly in HG group,while rosiglitazone inhibited those changes in dose-dependent manner.Conclusion Rosiglitazone can ameliorate the damage on the kidney fibroblasts induced by high glucose,thus have protecting effects on kidney.

Key concepts: Rosiglitazone, Endocrinology, Kidney, Internal medicine, In vitro, Messenger RNA, Chemistry, Diabetes mellitus

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