Effect of rosiglitazone on the expression of integrin β_1 in proximal tubular cell exposed to high glucose

ObjectiveTo evaluate the effect of rosiglitazone on the level of integrin β_1 in proximal tubular cells.Methods The proximal tubular cells of rats cultured in vitro were divided into 6 groups: control group,normal glucose group,high glucose group,high glucose with rosiglitazone of 5μmol/L group,high glucose with rosiglitazone of 10μmol/L group and single rosiglitazone of 10μmol/L group.The cells werecultured for 48 h.Integrin β_1 was detected by Western blot.Results The expression of integrin β_1 was significantly increased in high glucose group(84710.79±1810.44)as compared with control group(23283.17±827.55)and normal glucose group(47896.31±2014.63).Integrin β_1 in high(30 mmol) dose of glucose group with 5 or 10 mmol rosiglitazone was 31327.16±1721.36 and 38603.43±1409.30,respectively(both P0.005).Conclusion Rosiglitazone can strikingly inhibit the expression of integrin β_1 in proximal tubular cells exposed to high glucose with a marked dose-dependent manner.