Investigation on the Apoptpsis of Heptoma Cell Line HepG2 Caused by Allicin

Objective To investigate the effects of apoptosis on heptoma cell lines HepG2 caused by allicin.Methods CCK-8 was exerted to investigate the proliferation of the HepG2 cell and cytometry machine was used to detect the apoptosis and mortality rate,finally western blot method was used to study the apoptosis protein expression.Results Experimental results showed that allicin could inhibit the proliferation of cells and cause the cell apoptosis and death,and the highest ratio of apoptosis and death were 4.13% and 70.38%,respectively.Cell cycle experimental results indicated that the DNA metabolism was disturbance and most of them were blocked in G0/G1 period resulting in cells mitosis were blocked too.Western blot results showed that allicin the actived apoptosis gene of cells.Conclusion Allicin can inhibit the HepG2 cell proliferation and cause cell death and apoptosis,furthermore Allicin can cause cell DNA metabolism disturbance.Western blot experiments results show that the apoptosis gene can increase expression.