Investigate the effects of allicin on proliferation and apoptpsis of heptoma cell lines HepG2

Objective:To investigate the effects of allicin on proliferation of heptoma cell lines HepG2.Methods: Preparative HPLC was used to purify the allicin.Cell counting kit(CCK-8) was exerted to investigate the proliferation of the HepG2 cell and the flow cytometry was used to detect the apoptosis and mortality rate,finally Western blot method was used to study the expression of apoptosis protein.Results: Experimental results showed that allicin could inhibit the proliferation of cells and cause the HepG2 cells apoptosis and death,the highest ratios of apoptosis and death were 8.67%±3.2% and 70.38%±1.8%,respectively.The HepG2 cells′ DNA metabolism was disturbed and most of cells were blocked in G0/G1 period resulting in cells proliferation were blocked.Western blot results showed that the ratio of protein corresponding to apoptosis bax/ bcl-2 protein was increased indicating the cells was caused to apoptosis by allicin.Conclusion: Allicin could inhibit the HepG2 cell proliferation and cause cell death and apoptosis.Furthermore,allicin could also cause disturbance of HepG2 cell DNA metabolism disturbance,and the apoptosis protein increase its expression.