Research of Allicin's Anti-Cancer Effects on HepG-2 Cell Line

Objective:To investigate the anti-cancer effects of allicin on HepG-2 cell line by cell culture and try to find the related molecule mechanism of the effects.Method:HepG-2 cell line was taken as investigated subject,and the cell form's change was studied by invert microscope and hoechst stain invert fluorescence microscope after vary concentration allicin affected.MTT assay was used to study the cell's proliferative ability and calculate cell's inhibition ratio.The change of cell cycle and apoptosis rate were observed by flow cytometry.The change of the expression of Bcl-2,Bax and Caspase-3 were studied by immunocytochemical stain.Results:In test group,after 24 hours,HepG-2 cells ' form changed.The cells became more rounder than control group,and cells ' apophysis lost and distributing become very sparse.The nuclear concentrated and marginalized.The concentration of allicin were more higher and the changes became more clear.MTT assay demonstrated that the proliferation of HepG-2 cell can be inhibited by different concentrations allicin with different hours.Compared with negative control group and positive control group,the difference of the majority of test group had statistical significance.Flow cytometry showed that allicin group compared with negative group,the proportion of G0-G1 phase added and the cell apoptosis rate raised dramatically,and demonstrated the dose dependent manner.Immunocytochemical stain demonstrated that allicin can increase the expression of Bax and then the ratio of Bcl-2/Bax decreased and the expression of Caspase-3 was increased dramatically.Conclusion:Allicin can inhibit proliferation and induce apoptosis of HepG-2 cell in the certain scope.With the concentration of allicin raises,the proliferation was decreased and the apoptosis rate was increased,demonstrating the time-dose dependent manner.Allicin's effect of proliferation inhibition and apoptosis induction of HepG-2 cell is related to G0-G1 inhibition.The molecule mechanism of allicin's anti-cancer effects concern the increase of the expressions of bax and Caspase-3.