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2007•Journal of Bengbu Medical CollegeRequires access

Effect of growth and apoptosis on human leukaemia cells line HL-60 induced by allicin

Yuyun Li

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Abstract

Objective:To study the inhibitory effects of allicin and its induction of apoptosis on human leukemia cells HL-60.Methods:MTT was used to study the inhibitory effects of allicin on HL-60 cells growth and the typical morphology characters of apoptosis induced by allicin was observed with light microscope,and the apoptosis rate was analysed by flow cytometry(FCM).Results:MTT showed that HL-60 cells were suppressed with dose dependent relationship after exposed to allicin of 20,40,60 mg/L.Allicin induced apoptosis of HL-60 cells and apoptotic cells were supposed to be initiated in some phases of cell cycle by flow cytometry.Conclusions:Allicin can inhibit the growth rates of human leukemia cells HL-60 and induce its apoptosis.

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What this paper is about

Objective:To study the inhibitory effects of allicin and its induction of apoptosis on human leukemia cells HL-60.Methods:MTT was used to study the inhibitory effects of allicin on HL-60 cells growth and the typical morphology characters of apoptosis induced by allicin was observed with light microscope,and the apoptosis rate was analysed by flow cytometry(FCM).Results:MTT showed that HL-60 cells were suppressed with dose dependent relationship after exposed to allicin of 20,40,60 mg/L.Allicin induced apoptosis of HL-60 cells and apoptotic cells were supposed to be initiated in some phases of cell cycle by flow cytometry.Conclusions:Allicin can inhibit the growth rates of human leukemia cells HL-60 and induce its apoptosis.

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Available abstract

Objective:To study the inhibitory effects of allicin and its induction of apoptosis on human leukemia cells HL-60.Methods:MTT was used to study the inhibitory effects of allicin on HL-60 cells growth and the typical morphology characters of apoptosis induced by allicin was observed with light microscope,and the apoptosis rate was analysed by flow cytometry(FCM).Results:MTT showed that HL-60 cells were suppressed with dose dependent relationship after exposed to allicin of 20,40,60 mg/L.Allicin induced apoptosis of HL-60 cells and apoptotic cells were supposed to be initiated in some phases of cell cycle by flow cytometry.Conclusions:Allicin can inhibit the growth rates of human leukemia cells HL-60 and induce its apoptosis.

Key concepts: Allicin, Apoptosis, Flow cytometry, MTT assay, Medicine, Cell culture, Molecular biology, Leukemia

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