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2012•Strait Pharmaceutical JournalRequires access

Determination of carbamazepine concentration in human serum by HPLC

Hong Zhang

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Abstract

OBJECTIVE To establish an HPLC method for determining serum level of Carbamazepine.METHODS A symmetryshield RP18 column(5μm,3.9×150mm)was used.The mobile phase,methanol-phosphate solution(55∶45) was used at a flow rate 1.0mL·min-1,The detector is Waters 2487 Dual λ Absorbance Detector,detector was set at the wavelength of 210nm.RESULTS The calibration curve was linear in the range of 2.0 to 20.0μg·mL-1.The average recovery was 96%,and the RSD within-day and between-day were all less than 5%.CONCLUSION This method is suitable for therapeutic drug monitoring of Carbamazepine.

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What this paper is about

OBJECTIVE To establish an HPLC method for determining serum level of Carbamazepine.METHODS A symmetryshield RP18 column(5μm,3.9×150mm)was used.The mobile phase,methanol-phosphate solution(55∶45) was used at a flow rate 1.0mL·min-1,The detector is Waters 2487 Dual λ Absorbance Detector,detector was set at the wavelength of 210nm.RESULTS The calibration curve was linear in the range of 2.0 to 20.0μg·mL-1.The average recovery was 96%,and the RSD within-day and between-day were all less than 5%.CONCLUSION This method is suitable for therapeutic drug monitoring of Carbamazepine.

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Available abstract

OBJECTIVE To establish an HPLC method for determining serum level of Carbamazepine.METHODS A symmetryshield RP18 column(5μm,3.9×150mm)was used.The mobile phase,methanol-phosphate solution(55∶45) was used at a flow rate 1.0mL·min-1,The detector is Waters 2487 Dual λ Absorbance Detector,detector was set at the wavelength of 210nm.RESULTS The calibration curve was linear in the range of 2.0 to 20.0μg·mL-1.The average recovery was 96%,and the RSD within-day and between-day were all less than 5%.CONCLUSION This method is suitable for therapeutic drug monitoring of Carbamazepine.

Key concepts: Carbamazepine, Chromatography, Calibration curve, Chemistry, High-performance liquid chromatography, Absorbance, Phosphate buffered saline, Linear range

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