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2006•Strait Pharmaceutical JournalRequires access

Determination of Phenytoin Concentration in Human Serum by RP-HPLC

Huinan Wu

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Abstract

OBJECTIVE To establish an RP-HPLC method for determining serum level of Phenytoin. METHODS A symmetryshield RP_ 18 column (5μm,3.9×150mm) was used.The mobile phase,methanol-water (1∶1) was used at a flow rate 1.0mL·min -1 。The detector is Waters 2487 Dual λ Absorbance Detector,detector was set at the wavelength of 210nm.RESULTS The calibration curve was linear in the range of 2.0 to 40.0 μg·mL -1 .The average recovery was 98.23%,and the RSD within-day and between-day were all less than 3%.CONCLUSION This method is suitable for therapeutic drug monitoring of Phenytoin.

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What this paper is about

OBJECTIVE To establish an RP-HPLC method for determining serum level of Phenytoin. METHODS A symmetryshield RP_ 18 column (5μm,3.9×150mm) was used.The mobile phase,methanol-water (1∶1) was used at a flow rate 1.0mL·min -1 。The detector is Waters 2487 Dual λ Absorbance Detector,detector was set at the wavelength of 210nm.RESULTS The calibration curve was linear in the range of 2.0 to 40.0 μg·mL -1 .The average recovery was 98.23%,and the RSD within-day and between-day were all less than 3%.CONCLUSION This method is suitable for therapeutic drug monitoring of Phenytoin.

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Available abstract

OBJECTIVE To establish an RP-HPLC method for determining serum level of Phenytoin. METHODS A symmetryshield RP_ 18 column (5μm,3.9×150mm) was used.The mobile phase,methanol-water (1∶1) was used at a flow rate 1.0mL·min -1 。The detector is Waters 2487 Dual λ Absorbance Detector,detector was set at the wavelength of 210nm.RESULTS The calibration curve was linear in the range of 2.0 to 40.0 μg·mL -1 .The average recovery was 98.23%,and the RSD within-day and between-day were all less than 3%.CONCLUSION This method is suitable for therapeutic drug monitoring of Phenytoin.

Key concepts: Chromatography, Phenytoin, Calibration curve, Chemistry, Absorbance, High-performance liquid chromatography, Detector, Linear range

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