Determination of loganin and baicalin in Bushen Jiedu recipe by HPLC

Objective To establish the determination method of loganin and baicalin in Bushen Jiedu recipe by HPLC. Methods HPLC was used with Kromasil ODS-1 C18(5 μm,100mm×4.6 mm) as the analytical column,The flow rate was 1.0ml/min;①Baicalin:mobile phase was Methanol 0.1% Phosphate(42∶58).The UV detective wave length was at 280 nm.The sample volume was 5.0 μl.②Loganin: mobile phase was Pure water Acetonitrile(85:15),The UV detective wave length was at 236 nm.The sample volume was 10.0 μl. Results Baicalin showed good linear relationship(r=0.999 9)with in the range of 0.005~2.2 μg,and the average recovery rate was 98.18%(RSD=4.1%);Loganin showed good linear relationship(r=1.0)with in the range of 0.9996~199.92 μg,and the average recovery rate was 99.72%(RSD=2.0%). Conclusion The method is simple,accurate and durable for quantitative determination of of loganin and baicalin in Bushen Jiedu recipe.