Content Determination of Geniposide and Baicalin in Qingre Jiedu Soft Capsules by Dual Wavelength RP-HPLC

OBJECTIVE:To establish a method for the content determination of geniposide and baicalin in Qingre jiedu soft capsules.METHODS:HPLC method was adopted.The determination was performed on ZORBAX Eclipse XDB-C18 column with mobile phase consisted of acetonitrile(B)-0.2% phosphoric acid(C) by gradient elution at the flow rate of 0.4 mL·min-1(0～9 min,15%B～85%C) and 1.0 mL·min-1(10～20 min,25%B～75%C).The detection wavelength of geniposide and baicalin was set at 240 nm and 278 nm,respectively.The column temperature was maintained at 30 ℃ and injection volume was 10 μL.RESULTS:The linear range of geniposide and baicalin were 0.044～0.22 μg(r=0.999 8) and 0.56～2.8 μg(r=0.999 9),respectively.Their average recoveries were 98.55%,98.54%,98.56%;98.40%,98.41%,98.42%(RSD=0.919 7%,0.832 3%,0.859 6%;0.865 3%,0.805 8%,0.869 3%)(n=9),respectively.CONCLUSION:The method is simple,accurate,reproducible and precise,and it is suitable for quality control and evaluation of Qingre jiedu soft capsules.