HPLC-ELSD determination of jujuboside A and jujuboside B in different compatibility of Suanzaoren decoction

Objective: To establish an HPLC-ELSD method for determination of jujubosides A and B in different compatibility of Suanzaoren decoction.Methods: The assay was performed on a Grace Brava BDS C18(250 mm×4.6 mm,5 μm) column by a gradient elution program with acetonitrile-water as mobile phase at a flow rate of 1.0 mL·min-1,and the column temperature was 25 ℃.The evaporator tube temperature of ELSD was set at 100 ℃ with the nebulizing gas flow rate of 2.9 L·min-1.Results: The linear ranges of jujubosides A and B were 49.50-900.0 g·mL-1 and 20.40-407.2 g·mL-1,and the average recoveries(n=6) were 101.9%(RSD=2.3%) and 100.7%(RSD=3.0%),respectively Conclusion: When Ziziphi Spinosae Semen was combined with different herbs in Suanzaoren decoction,the contents of jujubosides A and B were changed.For this reason,the other herbs in decoction would evidently affect the amounts of jujubosides A and B released from Suanzaoren decoction.