Identification and Determination of Jujuboside A in Suanzaoren Hehuan Formula

Objective:To establish a TLC method for identification of Semen Zizyphi Spinosae and a HPLC-ELSD method for determination of jujuboside A in Suanzaoren Hehuan Formula.Method:Quality of Semen Zizyphi Spinosae was identified with thin layer chromatography.The analytical column was Ameritech Accurasil-ODS(4.6 mm × 250 mm,5 μm).The mobile phrase consist of methanol-water(60:40).The flow rate was 1.0 mL·min-1.The column temperature was set at 35 ℃.The tube temperature of the detector was 102 ℃.Result:TLC could be used for the identification of Semen Zizyphi Spinosae,the spots were clear and accurate.The jujuboside A was linear with-in the range of 0.6-3.0 μg(r = 0.999 9).The average recovery was 99.98% and RSD was 1.19%.Conclusion:These methods are sensitive,accurate and simple.The methods can be used for the quality control of Suanzaoren He-huan Formula.