HPLC-DAD-ELSD simultaneous determination of spinosin,jujuboside A and jujuboside B in Semen Ziziphi Spinosae(Suanzaoren)

Objective:To establish an HPLC-DAD-ELSD method for the simultaneous determination of spinosin,jujuboside A and jujuboside B in Semen Ziziphi Spinosae.Methods:The assay was performed on a Grace Brava C18-BDS(250 mm×4.6 mm,5 μm) column by a gradient elution program with acetonitrile-water as mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength of DAD was 335 nm and the evaporator tube temperature of ELSD was set at 100 ℃ with the nebulizing gas flow-rate of 2.9 L·min-1.The temperature of column was kept at 25 ℃.Results:The linear ranges of spinosin,jujuboside A and jujuboside B were 59.40-594.0 μg·mL-1(r=0.9996),49.50-900.0 μg·mL-1(r=0.9998),20.40-407.2 μg·mL-1(r=0.9999),respectively.The average recoveries(n=6) were 96.9%(RSD=1.8%),101.2%(RSD=2.5%),100.3%(RSD=2.9%),respectively.Conclusion:The method is accurate and reliable for simultaneous determination of multiple index components under the same chromatographic condition,and provides a reference for comprehensive quality evaluation of Semen Ziziphi Spinosae.